Dynamic remobilization of leaf nitrogen components in relation to photosynthetic rate during grain filling in maize

Abstract

Remobilization of leaf nitrogen (N) contributes greatly to grain N in maize, but leads to low photosynthetic rate (Pn). Pn is determined by various N components involving in light harvest and CO2 reduction. However, it is less clear which N component is the major contributor for the reduction of photosynthesis in modern stay-green maize hybrids. In this study, we analyzed the relationship between remobilization of different N components and Pn during grain filling stage under low N (no N application) and high N (180 kg N ha−1) in a field experiment. The remobilization efficiency of photosynthetic enzymes (PEPc, PPDK and Rubisco) in the leaf was much higher than that of thylakoid N and other N components. Low N supply increased the remobilization efficiency of all the leaf N components. During grain filling stage, the amount of all the N components decreased together with Pn. The ratio of Pn to the N in the PEPc, PPDK and Rubisco kept increase in the whole grain filling stage, while the ratio of Pn to chlorophyll and thylakoid-N decreased. Correlation analysis indicated that Pn was more related to the content of photosynthetic enzymes than to chlorophyll and thylakoid N. It is concluded that photosynthetic enzymes serve as an N storage reservoir at early grain filling stage and their degradation is critical in the reduction of Pn during later grain filling stage. Future breeding targets may be focused on enhancing the efficiency of photosynthetic enzymes during late grain filling stage.