Abstract
Proteins are dynamic and any detailed description of the proteome must reflect the dynamic variations in protein properties. For example, most proteins form complexes with other protein partners, can undergo various post translational modifications and can accumulate in different sub compartments of the cell. Spatial and temporal variations between proteins in different compartments and/or cell types mean that each experiment for mass spectrometric analysis must be carefully designed to optimise the data that can be obtained. Recent improvements in experimental methodologies and in the resolution and sensitivity of Mass Spectrometers, have expanded the complexity of proteomic analysis that is now possible[1]. In this chapter we outline current workflows and methodologies that facilitate complex proteomic analyses, from the design and execution of experiments, though to the analysis and interpretation of the resulting data.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
