Dynamic of Hepatitis E Virus (HEV) Shedding in Pigs.

Abstract

Simple SummaryHepatitis E virus (HEV) is an emerging pathogen, causing an increasing number of autochthonous cases in industrialized countries. In Europe, infections are associated with the zoonotic HEV-3 and HEV-4 genotypes and pigs and wild boars are the main reservoirs. A major concern of infections is linked to its foodborne transmission, due to consumption of raw or undercooked pork products infected by HEV-3 or HEV-4. HEV-3 is widespread in farmed pigs, mainly aged 3–4 months. Besides a decline with age, infected pigs have been observed at slaughterhouses, representing a risk for both the consumers and the workers of the pig industry. HEV is transmitted by the fecal–oral route and shed in feces in large amounts. The risk of viral spreading in farm and presence of infected pigs at slaughtering was evaluated by assessing the quantity and the duration of HEV-3 shedding in feces of infected pigs. Feces of 23 HEV-3 positive pigs were assayed during their fattening, shortly before their slaughtering. Results confirmed a long period of viral shedding in feces with a large amount of the virus released in the environment (mean 105 GC/g). Prevalence and quantity of the virus declines with the age of animals. The study provides information on the dynamic of the infection in pigs, important to prevent HEV occurrence and circulation in farms.Genotype 3 of hepatitis E virus (HEV-3) is the most common in Europe in both humans and pigs. HEV-3 strains are zoonotic, and foodborne cases associated with consumption of raw and undercooked pork products, mainly liver sausages, have been described. HEV-3 circulates largely in European pig farms, maybe due to its long persistence in the environment. Animals get infected around 3–4 months of age; shortly after, the infection starts to decline up to the age of slaughtering (8–9 months of age in Italy). With the purpose to understand the duration in farmed pigs of the shedding of the virus and its quantity, HEV-RNA detection was performed by Real-time RT-PCR from feces collected individually from two groups of 23 pigs. Sampling was conducted for 4 months shortly before slaughtering age. At 4-months-old, all animals were shedding HEV-3 to high load around 105 genome copies per gram (GC/g). Prevalence was higher in growers than in fatteners, with most of the pigs still positive around 166 days of age. Beyond some difference among individual pigs, the amount of HEV in feces decreased with the age of animals. The longest fattening period should ensure a lower risk of HEV shedder animals at slaughter, reducing the risk of food contamination.