Abstract
We demonstrated a real-time monitoring of live cells upon laminar shear stress stimulation via surface plasmon resonance (SPR) in gold nanoslit array. A large-area gold nanostructure consisted of 500-nm-period nanoslits was fabricated on a plastic film using the thermal-annealed template-stripping method. The SPR in the gold nanoslit array provides high surface sensitivity to monitor cell adhesion changes near the sensor surface. The human non-small cell lung cancer (CL1-0), human lung fibroblast (MRC-5), and human dermal fibroblast (Hs68) were cultured on the gold nanoslits and their dynamic responses to laminar shear stress were measured under different stress magnitudes from 0 to 30 dyne/cm2. Cell adhesion was increased in CL1-0 under shear flow stimulation. No adhesion recovery was observed after stopping the flow. On the other hand, MRC-5 and Hs68 decreased adhesion and recovered from the shear stress. The degree of recovery was around 70% for MRC-5. This device provides dynamic study and early detection of cell adhesion changes under shear flow conditions.
Highlights
Understanding mechanobiology is crucial to understanding the living creature
The medium of MEM supplemented with 3 mM Lglutamine, Earle’s balanced salt solution (EBSS) containing 1.5 g/ L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 10% fetal bovine serum (FBS) was for MRC-5 cell culture
We studied the dynamic responses of cells under different laminar shear stresses by using a gold nanoslit array surface plasmon resonance (SPR) sensor
Summary
Mechanical force induces structure change and changes the function of tissue [1]. There are some connate forces, such as blood pressure, breathing, bone support, and muscle contraction which complete the basic functions of life. Cells in the tissue are the basic unit as the reactor to the mechanical force. The functional expression of cells induced by the mechanical stimulation is regulated by the signaling cascades of gene expression and protein synthesis. It brings about cell grow, death, proliferation [2], differentiation [6] and tissue remodeling [4], which are important to tissue homeostasis. Abnormal mechanical stimulation alters the cellular function and the extracellular matrix (ECM) composition, leading to organ pathologies such as osteoarthritis, tendinopathy, and fibrosis in bone, vessels, heart, lung, and skin [5,7]
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