Abstract
Bacillus thuringiensis is a microorganism used for the production of biopesticides worldwide. In the present paper, different kinetic models were analyzed to study and compare three different strains of Bt ssp kurstaki (LIP, BLB1, and HD1). Bioperformances (vegetative cell, spore, substrate, and protein) and successive culture phases (oxidative growth, limitation and sporulation, and protein release) were depicted with an overarching aim to estimate total protein productivity, yield, and titer. In the end, two models were calibrated using experimental dataset (11 batches culture in 3 L bioreactor with semisynthetic medium), subsequently validated, and statistically compared. Both models satisfactorily followed the dynamics of the experimental data. Finally, a dynamic model was selected following the Akaike information criterion (AIC).
Highlights
IntroductionB. thuringiensis is a facultative anaerobic Gram-positive sporulating bacterium, frequently used in the production of some biopesticides and as a source of genes for transgenic expression in plants [1]
The parameters found were used to observe the behavior of the four state variables in two batches for the BLB1 strain and one batch for the HD1 and LIP strains
The bibliographic research allowed understanding of the context and the different phenomena involved in the study of the B. thuringiensis culture, such as the particular life cycle of these microorganisms and the importance of the endotoxins produced
Summary
B. thuringiensis is a facultative anaerobic Gram-positive sporulating bacterium, frequently used in the production of some biopesticides and as a source of genes for transgenic expression in plants [1]. It usually inhabits different environments, among which soil, settled dust, insects, water, and others have been identified [2]. The products based on B. thuringiensis (Bt) provide effective and environmentally benign control of several insects in agricultural, forestry, and disease-vector applications [3]. This insecticidal activity is mainly due to the production of some intracellular inclusions (called σ-endotoxins) during the sporulation phase of B. thuringiensis cells
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