Abstract
A dynamic microwave-assisted extraction (DMAE) method is established for the extraction of total ginsenosides from ginseng fibrous roots. The extraction process has been simulated and its main affection factors (liquid/solid ratio K of solvent to ginseng powders (V/m), irradiation time, irradiation temperature, extracting solution concentration, flow rate of solvent and microwave power) have been optimized by response surface methodology (RSM). The optimum conditions of extraction are the liquid/solid ratio of 270 mL/g, the extraction temperature of 75 °C, the extraction time of 35 min, ethanol concentration of 70% (V/V), the solvent flow rate of 1.3 mL/min, and the microwave power of 500 W. The yield of ginsenosides obtained by the proposed DAME method is (15.0±0.7) %, which is well agreement with the yield predicted by the model. Compared with static microwave-assisted extraction, DMAE has a higher extraction yield and can avoid the degradation of ginsenoside.
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