Abstract

Fast conformational changes of biological macromolecules such as RNA folding and DNA—protein interactions play a crucial role in their biological functions. Conformational changes are supposed to take place in the sub milliseconds to few seconds time range. The development of appropriate dynamic methods possessing both high space (one nucleotide) and time resolution is of important interest. Here, we present two different approaches we developed for studying nucleic acid conformational changes such as salt‐induced tRNA folding and interaction of the transcription factor NF‐κB with its recognition DNA sequence. Importantly, only a single laser pulse is sufficient for the accurate measuring the whole decay curve. This peculiarity can be used in dynamical experiments.

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