Abstract
This study relates to the mathematical modelling of enzymatic production of Cyclodextrins (CDs) by Cyclodextrin Glucanotransferase (CGTase) from Bacillus macerans. The experiments were carried out in batch mode using different starch sources and the results were used to estimate unknown parameters using linearization and dynamic simulation methods. ?- and ?-CD produced from tapioca were found to give the highest Michaelis-Menten constant, KM,i of 58.23 and 54.07 g L-1, respectively and maximum velocity, Vmax,i of 3.45 and 2.76 g L-1.min, respectively, while sago resulted in the highest KM,i and Vmax,i values of 342.35 g L-1 and 5.97 g L-1.min, respectively, for ?-CD obtained by the linearization method. Value of product inhibition, K1,i and CD degradation coefficient rate, ?CD,i, were estimated using dynamic simulation, indicating that exponential reaction kinetics could be fitted better with the experimental data. Sensitivity analysis revealed that the product inhibition parameter in the exponential reaction kinetic equation is more significant in the process. For validation, the production of CDs by fed batch method was undertaken and starch and enzyme were added into the reaction medium. Then, the predicted profiles generated by simulation were compared with the experimental values. The proposed exponential reaction kinetics shows good fitting with the experimental data.
Highlights
Within the cavity of their ring structure (Pedersen et al, 1995; Atasanova et al, 2009)
A mathematical model for the production of Cyclodextrin Glucanotransferase (CGTase) from native and immobilized Bacillus circulans strains had been developed by Burhan et al (2005), but the developed mathematical model focuses on the microbial CGTase production
The impact of the model on the endpoint CD production was investigated by increasing and decreasing 30% of Kl,i, which influences the rCD,i value
Summary
Within the cavity of their ring structure (Pedersen et al, 1995; Atasanova et al, 2009). Syahinaz Shahrazi et al / American Journal of Biochemistry and Biotechnology 9 (2): 195-205, 2013 potato, tapioca and corn have been widely used as substrates for the production of CDs. CGTase is active on both structures of amylose and amylopectin. In order to improve the bioprocess design of CD production, a kinetic model of enzymatic reaction studies is required (Vasic-Racki et al, 2003). Very limited information on reaction kinetic model of CGTase to produce CDs can be found. The aim of this study is to modify the kinetic model that can adequately describe the kinetic behaviour of α-, β- and γ-CD production by CGTase from B. macerans using different types of substrates (sago, corn and tapioca starch)
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