Dynamic levels of hepatocyte growth factor and vascular endothelial growth factor in the development of bronchopulmonary dysplasia

Abstract

Objective To investigate the dynamic levels of hepatocyte growth factor (HGF), vascular endothelial growth factor(VEGF) in bronchopulmonary dysplasia (BPD), so that to reveal the relationship between two growth factors and BPD. Methods Sixty preterm newborns ( 0.05). Bronchoalveolar lavage fluid(BALF) samples were collected from 60 ventilated preterm infants on 1st, 3rd, 7th, 14th day after birth. The levels of HGF, VEGF and secreted immunoglobulin (sIg) A were quantified by enzyme linked immunosorbent assay (ELISA), at the same time, clinical information were collected. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Jinhua Central Hospital and The 2nd Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University. Informed consent was obtained from the parents of each participating child. Results ①Ventilator therapy duration in BPD group was significantly longer than that in BPD-free group (Z=3.283, P=0.001). ②BPD group has lower levels of VEGF and HGF than those in BPD-free group at four different time-points (P<0.05), while the levels of VEGF became higher and higher, and the levels of HGF became lower and lower. The levels of HGF and VEGF on the 3rd day after birth showed negative connection with BPD(OR=0.806, 95%CI: 0.764~0.851, P<0.01; OR=0.288, 95%CI: 0.189~0.439, P<0.01). Conclusions Low levels of HGF and VEGF contribute to the development of BPD. The levels of VEGF and HGF on the 3rd day after birth are connected with the development of BPD. Key words: Bronchopulmonary dysplasia; Hepatocyte growth factor; Vascular endothelial growth factor; Infant, premature