Dynamic expression of proteoglycans during chicken skeletal muscle development and maturation

Abstract

Skeletal muscle development is a complex process in which cell migration and adhesion play important roles. Because these cellular activities involve cell surface and extracellular matrix molecules, proteoglycan analysis was performed for developing chick skeletal muscle. Proteoglycans are macromolecular conjugates of protein and carbohydrate found in the extracellular matrix and at the cell surface. In developing muscle, both in vivo and in vitro, there is a development-related progression from synthesis of primarily large proteoglycans at earlier stages to mainly small proteoglycans at later stages. This progression was demonstrated by radiolabeling developing muscle and extracting and characterizing the proteoglycans. The large proteoglycans synthesized earlier in myogenesis have been identified as the large chondroitin sulfate proteoglycan, versican. Among the small proteoglycans synthesized at later stages is the small dermatan sulfate proteoglycan, decorin. Immunolocalization of these proteoglycans shows that versican is initially present in pericellular locations around developing myotubes, whereas decorin is observed in the epimysium early in development, and then its distribution gradually spreads to also include the perimysium and endomysium. Studies of regenerating muscle show that there is a recapitulation of the embryonic pattern of proteoglycan synthesis, which, coupled with the results from embryonic muscle development, suggests a role for versican in some early aspect of myogenesis.