Abstract
Objective To observe the dynamic expression changes of microRNA(miR)-146a and miR-29 in neonatal rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS), and to explore their influence on ALI. Methods Totally 80 newborn rats aged 7 days were randomly assigned into the LPS group and control group.Intraperitoneal injection of endotoxin(5 mg/kg) was done to establish neonatal rat model of ALI in the LPS group.Rats in the control group were intraperitoneally injected with 0.1 mL 9 g/L saline.Lung tissue and blood specimens from newborn rats were taken at 6 hours, 1 day, 3 days and 7 days after treatment.Lung pathological changes were observed, and the levels of serum tumor necrosis factor-alpha (TNF- α) were detected by enzyme-linked immunosorbent assay.The relative expressions of lung tissues miR-146a and miR-29a were determined by reverse transcription-polymerase chain reaction. Results The lung tissue hematoxylin-eosin staining indicated ALI in the LPS group.Compared with the control group, the levels of serum TNF- α and lung tissue miR-146a showed high expression (P< 0.05), while the miR-29a was significantly reduced (P< 0.05). Conclusions Differential expressions of miR-146a and miR-29a have an important role in neonatal rat ALI.The key targets may provide new targets for early clinical diagnosis and treatment of ALI, as well as research and development of new medicine. Key words: Neonatal rat; Acute lung injury; MicroRNA-146a; MicroRNA-29a
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