Abstract
Dynamic cultivation of human stem cells under physiological conditions
Highlights
The development of stable tissue-engineered autologous bone grafts in the field of regenerative medicine is still a challenge
Cells were seeded on a three-dimensional macro-porous zirconium dioxide ceramic scaffold (0.3·106 cells/scaffold) and cultivated in standard growth medium (GM)or osteogenic differentiation medium (ODM) under normoxic (21% O2) or hypoxic (5% O2) conditions for a period of 21 days
Flow rates between 0.3 - 5 ml/min result in fluid shear stress between 0.01 - 2.5 Pa which is in the range of physiologic shear stress bone progenitor cells are subjected to in vivo (0.3 - 3 Pa) [2]
Summary
The development of stable tissue-engineered autologous bone grafts in the field of regenerative medicine is still a challenge. Perfusion bioreactors provide continuous nutrition supply and waste removal, but are suitable for the controlled application of mechanical forces like fluid shear stress. Mechanical loading is known to cause mechanotransductive effects like the induction of differentiation, resulting in enhanced deposition of extracellular matrix [1].
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