Dynamic comparison between endotoxemia and transforming growth factors after partial hepatectomy in rats

Abstract

Objective To compare and evaluate the dynamic changes between endotoxemia and transforming growth factors after partial hepatectomy(PH)in rats.Methods One-hundred and two adult male Wistar rats were randomized into three groups:A normal control (NC)group,a partial hepatectomized(PH)group and a sham-operated(SO)group.To study the dynamic changes of endotoxemia and transforming growth factors,the rats were sacrificed before and at different time points after partial hepatectomy(6,12,24,36,48,72,120 and 168 h).NC group was used as 0 h observation time point,namely 0 h group.For each time point indicated,six rats were used in parallel.Level of endotoxin(ET)was determined in serum by using Limulus Lysate test with chromogenie substrate.Levels of TNF-α and TGF-α were determined in serum by using RIA.Level of TGF-β1 was determined in serum by using ELISA.Results Serum ET levels significantly increased during 6-72 h after PH,there were two peak values at 12,48 h,and compared respectively with the ET levels of NC group (P＜0.05,P＜0.01).Serum TNF-α levels were transiently increased significantly compared with NC (P＜0.05)at 6 h after PH,then decreased rapidly to the lowest value at 72 h(P＜0.01).Serum TGF-α levels significantly increased during 6 h-72 h after PH.There was positive relationship be tween the dynamic changes of TGF-α and ET in serum(r=0.778,P＜0.05).Serum TGF-β1 levels were increased significantly compared with NC(P＜0.01)at 6 h after PH.There was positive rela tionship between the dynamic changes of TGF-β1 and TNF-α in serum(r=0.757,P＜0.01).Conclusion Endotoxemia and inflammatory mediators induced bv PH can regulate liver regeneration by influencing transforming growth factors which are related to hepatocyte proliferation. Key words: Hepatectomy; Endotoxemia; TGF-α; TGF-β1