Dynamic changes of the expressions of neuroserpin and tissue plasminogen activator during hypoxia/reoxygenation in primarily cultured rat cortical neurons

Abstract

Objective To investigate the ambulatory changes of the expressions of neuroserpin （NSP） and tissue plasminogen activator （tPA） during hypoxia/reoxygenation （H/R） in primarily cultured rat cortical neurons. Methods Cortical neurons were cultured from SD rats within 24 h after birth, and a H/R model was built. The immunofluorescence double staining and Western blot were used to detect the NSP expression in the cultured neurons. Enzyme-linked immunosorbent assay （ELISA） was used to detect the expression of tPA. Results After oxygenglucose deprivation （OGD） for 1.5 h, the expression of NSP protein was mildly up-regulated, and it reached the peak at 6 h after reoxygenation （P 〈 0.05）. Then it decreased slowly and basically restored to the level before hypoxia at 24 h after reoxygenation. The cells expressed a small amount of tPA at 1.5 h after OGD （P 〈 0.05 ）. With the extension of time for reoxygenation, the tPA content increased gradually. OGD was significantly higher at 6 h after reoxygenation than before （P 〈 0.01 ）. Then it decreased gradually, and it basically restored to the level before reoxygenation at 24 h after reoxygenation. Conclusions The expressions of NSP and tPA were up-regulated significantly during neuron H/R injury, and the ambulatory changes of NSP and tPA were basically the same. Key words: Neuropeptides; Tissue plasminogen activator; Hypoxia-ischemia, brain; Reperfusion injury; Neurons; Rats