Dynamic changes of nutrient content in herbaceous peony seed

Abstract

Herbaceous peony is a promising oilseed crop. ‘Hangshao’ and ‘Fenghuang Niepan’ are two cultivars with high yield and oil content. In this study, seed reserved nutrient of these two genotypes were investigated during seed development process. Results showed that ‘Hangshao’ and ‘Fenghuang Niepan’ matured approximately 85 ​d after flowering (DAF). The development process could be divided into four stages: seed formation and enlargement stage (S1), seed inclusion and enrichment stage (S2), crude fat rapid accumulation stage (S3) and dehydration and color transition stage (S4). During this process, 100-seed fresh weight (HFW) and 100-seed dry weight (HDW) increased, whereas water content (WC) decreased. HFW, HDW and WC for ‘Hangshao’ seed at 85 DAF were 28.56 g, 20.78 ​g and 37.50% respectively, whereas those for ‘Fenghuang Niepan’ seed were 27.2 ​g, 19.78 ​g, and 30.85% respectively. In terms of the primary metabolites measuring, soluble protein content was consistently low (only 2.24%–3.15%), while starch content was relatively high (5.60%–24.81%). They displayed a trend of gradual increasing in early stage, followed by rapid decline. Crude fat tended to accumulate continuously with a linear upward trend starting at 40 DAF, and its maximum level was reached at 85 DAF (27.57% for ‘Hangshao’ and 29.57% for ‘Fenghuang Niepan’). For the secondary metabolites determination, total phenols content was relatively steady, maintaining at 107.7–129.9 ​mg/g for both cultivars, while total flavonoids content varied within 25.33–65.33 ​mg/g for ‘Hangshao’ and 24.33–52.37 ​mg/g for ‘Fenghuang Niepan’. The maximum level for both cultivars occurred at 55 DAF. Furthermore, crude fat content was found to be highly correlated with WC by correlation analysis and linear regression model definition (P ​≤ ​0.01 and r ​= ​0.954). Thus, WC was recommended for evaluating crude fat in peony seed. These findings could provide scientific reference for high-yielding production practice for oleaginous peony plant.