Dynamic changes of intracellular Ca2+ content of rats brain after traumatic brain injury and its correlation with the concentration of calcitonin gene‐related peptide

Abstract

ObjectiveTo investigate the dynamic changes of intracellular Ca2+ content of rats brain after traumatic brain injury (TBI) and its correlation with the concentration of calcitonin gene‐related peptide (CGRP)MethodsThe rats model with TBI was prepare by Feeney’s methods. 144 male Wistar rats were randomly divided into Sham operation group(Sham group, 36 rats) and traumatic brain injury group (TBI group, 108 rats ). Above two groups subdivided into six groups by 2h,6h,12h,24h, 48h and 72h after TBI( 6 rats in each group. The calcium content in the rats brain cells were detected by laser scanning confocal microscope, and the CGRP concentration in plasma of rats were got by enzyme‐linked immunosorbent assay.ResultsThere were significant differences of calcium fluorescence intensity in the rats brain cells between the Sham group and the TBI group (P<0.05). In the meantime there were also significant differences of the CGRP concentration in plasma of rats between the Sham group and the TBI group (P<0.05). After the injury, calcium fluorescence intensity in the rats brain cells began to rise within 2 hours, reached its peak in 24 hours, and decreased in 48 hours (P<0.05). In the TBI group, the CGRP concentration in plasma of rats increased at 2h, then decreased at 24h,48h, and increased slowly at 72h after TBI (P<0.05).ConclusionThere was a negative correlation between CGRP concentration in rats plasma and the intracellular calcium content of rats brain. Calcium overload played an important role in the brain secondary damage after TBI.Support or Funding Information• This study was supported by the National Natural Science Foundation Youth Fund (30600637), China Postdoctral Science Foundation Grant (2019T120195), China Postdoctral Science Foundation Grant (2014M561207), the Shanxi Province Basic Research Program ( 2010021034‐4, 201601D011119), the Shanxi Scholarship Council of China (2011‐096 and Key Projects No.4), Department of Human Resources and Social Security of Shanxi province fund grant, The science and technology research projects of health department of Shanxi Province(2016D011119), The Teaching Reform Program of Shanxi Higher Education, The Education Reform Project of Postgraduate Students in Shanxi Province. The Project of “131” Leading Talents of Shanxi Higher Education, The Education Reform Project of Shanxi Medical University, the fund grant of the eleventh college of shanxi medical university(Lvliang Hospital).