Abstract
To better understand the regulatory mechanism of phenolics and ascorbic acid accumulation as well as antioxidant activities in mung beans during legume development, the gene expression profiles of 25 key-coding genes in ascorbic acid and phenolics metabolic pathways were analyzed. As well as the dynamitic changes of ascorbic acid, phenolic profiles and antioxidant activities with legume development were studied. The results indicated that gene expression profiles were closely related to the ascorbic acid and phenolics accumulation regularity during legume development. VrVTC2 and VrGME played important roles for ascorbic acid accumulation from 8 to 17 days after flowering (DAF). VrPAL and VrCHS exhibited positive correlations with daidzein and glycitin accumulation, and VrIFS had a strong positive correlation with glycitin biosynthesis. Antioxidant activities dramatically increased during mung bean maturing, which were significantly related to ascorbic acid and phenolics accumulation. Eight days after flowering was the essential stage for ascorbic acid and phenolics biosynthesis in mung beans.
Highlights
Previous studies have shown that regular consumption of fruits and vegetables lowers the risk of chronic diseases [1,2]
There was a significant time-dependent increase in ascorbic acid content until it reached the highest value on 17 days after flowering (DAF), in which the peak value was 19.91 ± 1.70 mg/
Ascorbic acid content decreased on 11 and 14 DAF, it generally showed an upward trend during mung bean legume development
Summary
Previous studies have shown that regular consumption of fruits and vegetables lowers the risk of chronic diseases [1,2]. Gene expression profiles in the ascorbic acid metabolic pathway are closely related to changes of ascorbic acid accumulation [13]. It has been reported in the literature that GDP-L-galactose phosphorylase (VTC2) plays a key regulatory role in ascorbic acid biosynthesis [14,15]. Many investigations have focused on phenolics, ascorbic acid and antioxidant activities in mung beans [3,5]. The intention of this study is to evaluate the related gene expression profiles of phenolics and ascorbic acid metabolic pathways, determine the accumulation of phenolics and ascorbic acid, and investigate antioxidant activities in mung beans during legume development
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