Abstract
Objective To investigate the dynamic changes in myocardial microRNA (miRNA) expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest.Methods Sixty-four adult male Sprague-Dawley rats,weighing 250-330 g,were randomly divided into 2 groups (n =32 each):control group (group C) and cardiopulmonary resuscitation after cardiac arrest group (group CA-CPR).The rats were anesthetized with intraperitoneal chloral hydrate 7 ml/kg,tracheostomized and mechanically ventilated.The model of cardiac arrest was established by clamping the endotracheal tube at end expiration and cardiopulmonary resuscitation was performed.At 3,6,12 and 24 h after recovery of spontaneous circulation (T1-4),blood samples were collected for determination of serum creatine-kinase isoenzyme MB (CK-MB) and cardiac troponin I (cTnI) concentrations and myocardial specimens were obtained from the apex for detection of the expression of miRNAs in myocardial tissues (by using RT-PCR).Results Compared with group C,serum CK-MB and cTnI concentrations were significantly increased at each time point in group CA-CPR (P ＜ 0.01).The serum CK-MB concentration reached the peak at T3,and the serum cTnI concentrations continued to increase in group CA-CPR (P ＜ 0.05 or 0.01).There were 8 miRNAs which changed continuously after recovery of spontaneous circulation,and there were miRNA-1,miRNA-21,miRNA126,miRNA-133a,miRNA-206,miRNA-210,miRNA-320 and miRNA-499.The expression of miR-NA-1 was up-regulated at each time point and peaked at T2.The expression of miRNA-21 was down-regulated at each time point and reached the lowest level at T2.The expression of miRNA126,miRNA-133a,miRNA-206,miRNA-210 and miRNA-499 was down-regulated at T1 and T2,was up-regulated at T3 and T4,and reached the lowest level at T2.The expression of miRNA-320 was up-regulated at T1-T3,was down-regulated at T4,and peaked at T2 (P ＜ 0.05 or 0.01).Conclusion Eight miRNAs exhibit changes in expression after cardiopulmonary resuscitation in a rat model of asphyxial cardiac arrest and the way and tendency that miRNAs have changed are different,but the time point at which the way that miRNAs have changed is similar. Key words: Heart arrest ; Cardiopulmonary resuscitation ; MicroRNAs
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
