Abstract
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.
Highlights
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy
We report for the first time the non-contact and real-time measurement of the shear wave velocity and elastic modulus during the dynamic blood coagulation process using the ARFOE-Optical coherence elastography (OCE) method, where the acoustic radiation force (ARF) orthogonal to the optical coherence tomography (OCT) beam produces the vibration of the blood tissue and the shear wave propagation is visualized utilizing phase-resolved OCT detection with the Doppler variance method
For the shear wave detection, 43 M-scans are captured at the position indicated by the white arrow in Fig. 1a using each of three ARF excitation positions with a spacing of 1 mm
Summary
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. We report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. MMOCE method uses fundamental resonant frequency to represent the elastic properties of clotted blood It cannot monitor the dynamic change of the elastic properties during blood coagulation and needs contact between microbead and detected sample for the magnetic force application, which may change the coagulation process. The shear wave velocity V can be directly used to calculate the shear modulus ΞΌ by the following equation:
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
