Abstract
We report single-molecule force spectroscopy experiments on double-stranded DNA using a dynamically oscillating AFM cantilever driven in a frequency modulation mode with a constant excitation (CE) amplitude. While the DNA molecule is stretched, we measured the shift of the cantilever amplitude and the resonance frequency and estimated both the conservative forces and the dissipated energy. Typical elastic features of the calculated force distance curves are in nice agreement with data known from quasi-static stretching experiments. Analysis of the data indicates that the melting dynamics of the DNA base pairs depends on the amount of already melted base pairs and the base pair recombination rate. Furthermore, we observe a reduction of the molecular elasticity modulus, which is most likely caused by the cantilever dynamics leading to a destabilization of stacking and hydrogen bond interactions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.