Dyella marensis sp. nov., isolated from cliff soil

Abstract

A novel, Gram-negative bacterium, designated CS5-B2(T), was isolated from soil that had been collected from a cliff on Mara Island, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the radiation of the genus Dyella. The closest relatives were the type strains of Dyella koreensis, Dyella ginsengisoli and Dyella japonica and 16S rRNA gene sequence similarities between strain CS5-B2(T) and these strains were 99.0, 97.9 and 97.8 %, respectively. The sequence similarities between the novel isolate and other related taxa compared in the phylogenetic analysis were less than 96.7 %. The cells of strain CS5-B2(T) were aerobic, oxidase-negative, catalase-positive, motile rods. The temperature range for growth was 20-37 degrees C, with optimal growth at 30-37 degrees C. Growth occurred at pH 5.1-9.1, with optimal growth at pH 6.1-9.1. NaCl tolerance for growth was from 1 to 2 % (w/v). Ubiquinone-8 was the predominant respiratory lipoquinone. The major fatty acids were iso-C(15 : 0) and iso-C(17 : 1)omega9c. The G+C content of the DNA was 65.7-66.6 mol%. The level of DNA-DNA relatedness with D. koreensis KCTC 12359(T) was 20.2 and 29.6 % in duplicate measurements. On the basis of phenotypic features, phylogenetic analysis and DNA-DNA relatedness, a novel species of the genus Dyella is proposed, with the name Dyella marensis sp. nov. The type strain is CS5-B2(T) (=JCM 14959(T) =KCTC 22144(T)).