Abstract
Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel hikes. This study was conducted with the concept of implementing an effluent wastewater as lipid production medium for microalgae cultivation. In our study textile dyeing industry effluent was taken as a lipid production medium for Neochloris sp. cultivation. The changes in physicochemical analysis of effluent before and after Neochloris sp. treatment were recorded using standard procedures and AAS analysis. There was especially a reduction in heavy metal like lead (Pb) concentration from 0.002 ppm to 0.001 ppm after Neochloris sp. treatment. Neochloris sp. cultivated in Bold Basal Medium (BBM) (specific algal medium) produced 41.93% total lipid and 36.69% lipid was produced in effluent based cultivation. Surprisingly Neochloris sp. cultivated in effluent was found with enhanced neutral lipid content, and it was confirmed by Nile red fluorescence assay. Further the particular enrichment in oleic acid content of the cells was confirmed with thin layer chromatography (TLC) with oleic acid pure (98%) control. The overall results suggested that textile dyeing industry effluent could serve as the best lipid productive medium for Neochloris sp. biodiesel feedstock preparation. This study was found to have a significant impact on reducing the biodiesel feedstock preparation cost with simultaneous lipid induction by heavy metal stress to microalgae.
Highlights
Algae comprise a wide variety of photosynthetic heterotrophic organisms that belong to different phylogenetic groups, denoting many taxonomic divisions
The obtained Neochloris sp. was initially cultivated in 200 mL Bold Basal Medium (BBM) (Figure 1) and kept at 25∘C under 1500 Lux illumination for 21 days, and subsequent microscopic observations were performed to know the purity of the strain
This study was started with the concept of implementing textile dyeing industry effluent as lipid production medium for microalgae
Summary
Algae comprise a wide variety of photosynthetic heterotrophic organisms that belong to different phylogenetic groups, denoting many taxonomic divisions. They are inhabited predominantly in fresh and marine water ecosystems. Among them microalgae are predominant in both marine and freshwater ecosystems. They contribute to more than half of the total primary production at the bottom level of the food chain [1]. Most of the microalgae can survive and proliferate in a wide range of environmental conditions due to their secondary metabolite acquisition. Microalgae gained more attention in the food, aquaculture, fuel, and biotechnology industries [2]
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