Dye Release Experiments with Dextran Loaded Vesicles

Abstract

Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously encapsulating dye of different sizes, such as dextrans grafted with a chromophore. In this assay, dextran linked to rhodamine or fluorescein are both encapsulated in lipid vesicles to allow quantifying the leakage of each dextran individually from a single sample. For instance, the size evaluation of the lipid pore formed by an antimicrobial peptide has been recently achieved using this protocol (Sani et al., 2013)., 染料释放实验是一种广泛使用的方法来评估膜 - 活性分子和脂质膜之间的相互作用。 特别感兴趣的是通过同时包封不同尺寸的染料（例如用发色团接枝的葡聚糖）来评估脂质双层扰动的程度的能力。 在该测定中，连接到罗丹明或荧光素的葡聚糖都封装在脂质囊泡中，以允许从单个样品单独地量化每个葡聚糖的渗漏。 例如，最近使用该方案获得了由抗微生物肽形成的脂质孔的尺寸评价（Sani等人，2013）。