Dye-marked paraventricular neuroendocrine cells in vivo in cat hypothalamus

Abstract

In antidromically identified neurons in the cat hypothalamus we recorded and injected fluorescent dye-markers (Lucifer Yellow, LY; Procion Yellow, PY) intracellularly. The dye-filled neurons lay in the rostral portion of the magnocellular paraventricular nucleus of the hypothalamus. We observed two morphological cell types of similar size based on the intracellular injections of LY or PY: a bipolar cell type with fusiform perikaryon and a multipolar cell type with a polygonal perikaryon. These morphological cell types correspond to previous descriptions of immunocytochemically identified vasopressinergic and oxytocinergic magnocellular neurons in mammals. This study demonstrates the feasibility of in vivo intracellular dye-marking and electrophysiological recordings from mammalian hypothalamic neurons. We have here a basis for correlating morphological characteristics with the physiological traits of single magnocellular neuroendocrine cells.