Dye Amount Estimation in a Papanicolaou-stained specimen using Multispectral Imaging

Abstract

Papanicolaou stain is mainly used in cytological diagnosis such as gynecological diseases. In the image analysis of stained histopathology specimens, color unmixing technique, which estimates the dye abundance map, is useful. In this paper, we apply the dye amount estimation method based on color unmixing to Papanicolaou-stained specimen. In the proposed method, we capture the Papanicolaou-stained samples using a multispectral microscope, and then we estimate the amount of dyes from the observation and practically measured the spectral characteristics of the stain. Besides, we construct an application depicting the amount of stain and the bar-graph plot. In the experiments, we verify the feasibility of the proposed method and analyze a precancerous lesion of the uterine cervix using the proposed method.