Abstract
Polyacrylonitrile (PAN) nanofibrous membrane was prepared by an electrospinning technique. After heat treatment and alkaline hydrolysis, the weak ion exchange membrane was grafted with chitosan molecule and then covalently immobilized with a Cibacron Blue F3GA (CB). Fibre diameter, porosity and pore size of the membrane and immobilized dye density were characterized. Furthermore, the membrane was applied to evaluate the binding performance of lysozyme under various operating parameters (pH, chitosan mass per volume ratio, dye concentration, ionic strength and temperature) in batch mode. The experimental results were directly applied to purify lysozyme from chicken egg white by membrane chromatography. The results showed that the capture efficiency, recovery yield and purification factor were 90 and 87%, and 47-fold, respectively, in a single step. The binding capacity remained consistent after five repeated cycles of adsorption-desorption operations. This work demonstrates that the dye-affinity nanofibrous membrane holds great potential for purification of lysozyme from real feedstock.
Highlights
Various nanofibrous membranes can be prepared by using an electrospinning process and they have been widely applied in diverse fields, such as tissue engineering [1,2], drug delivery [3], biosensors [4], antibacterial membranes [5], filtration and adsorptive membranes [6,7], and wastewater treatment [8]
Lysozyme was chosen as the model protein, and the dye-affinity nanofibrous membrane was used to assess the feasibility of the specific adsorption of protein under various operating parameters
It is well known that the morphology, mechanical properties and surface characteristics of the PAN nanofibre are significantly influenced by the polymer solution properties, processing parameters, and environmental conditions [29]
Summary
Various nanofibrous membranes can be prepared by using an electrospinning process and they have been widely applied in diverse fields, such as tissue engineering [1,2], drug delivery [3], biosensors [4], antibacterial membranes [5], filtration and adsorptive membranes [6,7], and wastewater treatment [8]. Column chromatography is an effective technique for purification of proteins. There are many disadvantages, such as compressibility of the packed beds, fouling of the adsorbent beads, and slow operating flow rate. To overcome these problems, membrane chromatography may be an alternative technique [9,10,11,12]. Nanofibrous membranes designed for use in purification of proteins should feature high porosity, large specific area, high chemical, biological and mechanical stabilities, high hydrophilic properties, low non-specific adsorption, and fast binding kinetics [6,10,12]. The high specific surface gives the nanofibrous membrane higher adsorption capacity and a faster adsorption rate than conventional membranes
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