Dwarfing genes and cell dimensions in different organs of wheat

Abstract

A field experiment was conducted under non-limiting water and nutritional conditions with three near-isogenic lines of spring wheat (dwarf, DD; semi-dwarf, SD and standard height, SH) to study the impact of the GA-insensitive alleles Rhtl and Rht2, at the cellular level, on the growth of different vegetative organs and of the pericarp of grains. Cell length and width of blades of different leaves (3, 7 and flag leaf), the flagleaf sheath and the penultimate internode as well as the pericarp of basal grains from central spikelets of the spike were evaluated. With the exception of the flag leaf, dwarfing genes produced a significant reduction in cell length in all the different vegetative organs analysed. There was no effect on the number of cells nor their width. Therefore, in vegetative organs, the effects of these alleles appeared to be exclusively due to a reduction in cell length. It would appear that dwarfing genes act on cell elongation without affecting cell division. The Rht alleles did not modify cell length nor width in the pericarp. Grain weight was different between the lines and these differences were associated with grain volume at the beginning of linear grain growth. Thus, they reduced the size of individual grains by reducing the total number of cells in the pericarp. It appears that Rht alleles reduced the final sizes of vegetative organs (such as internodes and leaves) and of tissues (pericarp) associated with reproductive structures (grains), but the modes of action in these different organs were different.