DUS Test and DNA Fingerprinting Construction of Jute Varieties

Abstract

AbstractIn order to promote the application of distinctness, uniformity and stability (DUS) test guidelines for jute and to promote the testing and identification of new jute varieties in this chapter, the details of DUS test guidelines were described, including the selection and determination of tested traits, the selection of standard varieties, and the evaluation of distinctness, uniformity and stability. There were 35 standard varieties and 33 tested traits in the DUS test, including 7 qualitative traits (QL), 18 quantitative traits (QN), 8 pseudo qualitative traits (PQ). Among the 33 tested traits, 21 characters with an asterisk were required to be tested, while 12 tested traits without an asterisk were selected. 5 tested traits were selected for variety grouping and 12 traits were used in the technical questionnaire. The formulation of jute DUS testing guidelines is helpful to strengthen the protection of new varieties, protect the interests of breeders and improve the enthusiasm of breeding. Furthermore, the applied core collection in jute was constructed, including 61 accessions divided into 16 applications-oriented features, such as high yield, high quality, and disease resistance and so on, which were established based on the field performance from 300 jute germplasm. 12 fluorescent core primers were screened from 46 pairs of core primers. Fluorescence-labeled capillary electrophoresis was used to analyze the polymorphism of these 12 pairs of primers, and a total of 140 polymorphism sites were detected. The precise DNA molecular ID cards were constructed by the combination of the 12 core primer pairs from the data coded in the form of numbers and English letters. Bar code and quick response code DNA molecular were established and can be quickly scanned and recognized by electric gadget. These results could be beneficial to increase the application efficiency and rapid molecular identification in jute germplasm resources.KeywordsJuteVariety selection and determinationDUSApplied core collection of germplasmSSRDNA molecular identification