Durum wheat-lentil relay intercropping enhances soil mycorrhizal activity but does not alter structure of arbuscular mycorrhizal fungal community within roots

Abstract

In intercropping systems, crop species select host-adapted microorganisms and influence the associated plant-microbial interactions like in the case of arbuscular mycorrhizal fungi (AMF). Attempts to assess the impact of intercropping on the activity, diversity, and community composition of AMF remain inconclusive, more so in intercropping systems involving traditional Mediterranean crops such as durum wheat and lentils. We carried out field experiments in Central Italy to assess the impact of relay intercropping durum wheat (Triticum durum Desf. cv. Minosse) and lentil (Lens culinaris Medik. cv. Elsa) on soil mycorrhizal inoculum potential (MIP) (2019 and 2020), AMF root colonization (2019, 2020, and 2021), and root AMF diversity and community composition (2020 and 2021), compared to the respective sole crops. Results showed that relay intercropping enhanced lentil grain yield and durum wheat grain protein concentration but marginally reduced durum wheat grain yield and lentil grain protein concentration. In addition, relay intercropping enhanced soil mycorrhizal activity but differentially influenced mycorrhizal root colonization compared to sole cropping. Sequencing analyses generated a total of 234 amplicon sequence variants belonging to Glomeromycota, which were assigned to 31 virtual taxa using the MaarjAM reference database. Glomeraceae and Claroideoglomeraceae were the most abundant taxa but had contrasting abundances in 2020 and 2021. The overall changes in AMF species diversity and community structure were affected by the interaction between crop species and year, and not by intercropping. Claroideoglomus and Septoglomus showed a strong association with lentil roots while Rhizophagus and Paraglomus were associated with durum wheat roots in 2020, affirming host genotype-AMF preferences. The principal component analysis showed that grain protein concentration was associated with selected mycorrhizal parameters such as community richness and AMF root colonization. Further studies on the functional analysis of the different AMF communities selected by the crop genotype and year may reveal the importance of intercropping in maintaining soil functionality and productivity under low-input systems.