Abstract

The rate of formation of the crystals is the purpose of the present work in which the turnover time of the epithelium is evaluated by measuring the time required for migration of labelled cells from the crypts to the lining of the midgut. In insects killed a few hours after a tritiated thymidine injection, only the replacement cells of the crypts are labelled. If the insects are kept alive longer, the labelled replacement cells progressively migrate; 4 days after the injection all the nuclei of the midgut epithelium are labelled and notably the crystal-containing nuclei; therefore the whole epithelium has been renewed. Since the replacement cells of the crypts never contain crystals, these latter have been formed in less than 4 days.

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