Duration Exposure effect of Anti Mosquito Coils smoke on Lung and Microscopic condition of Heart and Haemoglobin in Mice (Mus musculus)

Abstract

The use of mosquito coils for a long time will harm the body, including the lungs and heart. Therefore, this study aims determine the microscopic condition of the lungs and heart and hemoglobin levels. The design of this study was a laboratory experiment that used sample of 24 mice divided into 4 groups, named P0(control), P1(4 hours), P2(6 hours), and P3(8 hours) for 30 days. Then the hemoglobin level was measured using the POCT method. After that, the mice were necropsied the observe the condition of the lungs and heart which had been stained with HE. Observations were conducted inscoring and process data using statistical test of one way ANOVA, if the data are not qualified, then Kruskal-walis test is used The results showed that the degree of lung damage obtained a score of P0, P1, P2, and P3 of 1.5;2;2,167; and 2,667. While the degree of hearth damage P0, P1, P2, and P3 obtained score of 0.5;1.5;1.3; and 1.67. The results of hemogobin levels (g/dl) showed that P0, P1, P2, P3 of 14.61; 17.4; 15.05; and 17.4. Microscopically, lung cell tissue was damaged when exposed to mosquito coil smoke, it is the presence of pycnotic nuclei in P2 and P3 treatments. The results of One Way ANOVA test on hemoglobin levels of mice showed significant results (p>0.05), while microscopically, heart cells using the Kruskal Wallis test showed significant differences (p>0.05). The conclusion of this study is that there is a significant chane between the leghth of time of exposure to mosquito coils with lung microscopy and the level of mice. However, there were no significant changes in the microscopic heart of mice.