Abstract
Objective: The study has been conducted to search out the threshold duration of treatment of ethyl acetate fraction of methanolic extract of leaves of Camellia sinensis (L.) Kuntze at the dose of 100 mg/kg body weight for the management of diabetes-induced testicular impairment in streptozotocin-induced diabetic rat in a duration dependent fashion.
 Methods: In this respect, the glycemic, androgenic, oxidative stress sensors, gene expression of testicular androgenic key enzymes along with apoptotic markers were evaluated in a duration dependent way (14, 28 and 56 d).
 Results: A significant correction was noted in the levels of glycated haemoglobin (HbA1C), testicular thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), sperm viability, sperm mitochondrial status, serum testosterone, and genomic expression of testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, Bax, Bcl-2 after treatment for different duration with the said fraction in diabetic groups in compare to respective vehicle-treated diabetic group without any toxicity induction in general. Thin layer chromatography (TLC) study of the fraction showed two spots with retention factors (Rf) of 0.78 and 0.51.
 Conclusion: The results showed that 28 d treatment was threshold duration of treatment for the correction of diabetes-induced testicular impairment.
Highlights
Diabetes mellitus is a metabolic disorder resulting from a defect in insulin secretion, insulin action, or both [1]
Administration of ethyl acetate fraction of methanolic extract of C. sinensis at the dose of 100 mg/kg body weight in 2 ml of distilled water two times/day for the duration of 14 or 28 or 56 d to diabetic rats, a significant diminution (p
A significant elevation (p
Summary
Diabetes mellitus is a metabolic disorder resulting from a defect in insulin secretion, insulin action, or both [1]. The pathology of diabetes mellitus is caused by Reactive Oxygen Species (ROS) that activate the non-enzymatic glycation of proteins which leads to structural and functional changes in proteins, DNA and lipid. Many studies have been focused that streptozotocin (STZ) induced diabetes in male rat resulted diminution in the activity of sex organs, low sperm count along with a low level of testosterone [4, 5]. Plant-derived preparations have been used as traditional remedies and in folklore medicine for the treatment of diabetes in many parts of the world [8, 9]. Traditional medicinal herbs are well accepted by the World Health Organization (WHO) expert committee to treat the diabetes mellitus due to its minimum side effect, low cost and easy availability [10, 11]
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