Abstract
Institutes of Histology and General Embryology of the University of Rome and of the School of Medicine of L'Aquila, Italy (Received 5th August 1974) Microdensitometric studies of DNA content in Feulgen-stained mouse zygotes (Alfert, 1950) have shown that the pronuclei duplicate their DNA before the prophase of the first cleavage division. The earliest incorporation of labelled adenine into DNA was autoradiographically detected by Sirlin & Edwards (1959) 13 hr after ovulation; according to Mintz (1965), DNA synthesis takes place by the end of the first third of the cycle. In the present study, we have investigated the timing of DNA duplication in the mouse zygote. While we were analysing our data, Luthardt & Donahue (1973) published an extensive work on pronuclear DNA synthesis in the mouse. Their findings will be discussed. Virgin Swiss female mice (CD-I COBS) purchased from Charles River Italia were kept in a room with controlled temperature
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