Duplication, divergence and persistence in the Phytochrome photoreceptor gene family of cottons (Gossypium spp.)

Ibrokhim Y Abdurakhmonov,Alan E Pepper,Carla Jo Logan-Young,Zabardast T Buriev,Abdusattor Abdukarimov

doi:10.1186/1471-2229-10-119

Ibrokhim Y Abdurakhmonov, Alan E Pepper + Show 3 more

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https://doi.org/10.1186/1471-2229-10-119

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Abstract

BackgroundPhytochromes are a family of red/far-red photoreceptors that regulate a number of important developmental traits in cotton (Gossypium spp.), including plant architecture, fiber development, and photoperiodic flowering. Little is known about the composition and evolution of the phytochrome gene family in diploid (G. herbaceum, G. raimondii) or allotetraploid (G. hirsutum, G. barbadense) cotton species. The objective of this study was to obtain a preliminary inventory and molecular-evolutionary characterization of the phytochrome gene family in cotton.ResultsWe used comparative sequence resources to design low-degeneracy PCR primers that amplify genomic sequence tags (GSTs) for members of the PHYA, PHYB/D, PHYC and PHYE gene sub-families from A- and D-genome diploid and AD-genome allotetraploid Gossypium species. We identified two paralogous PHYA genes (designated PHYA1 and PHYA2) in diploid cottons, the result of a Malvaceae-specific PHYA gene duplication that occurred approximately 14 million years ago (MYA), before the divergence of the A- and D-genome ancestors. We identified a single gene copy of PHYB, PHYC, and PHYE in diploid cottons. The allotetraploid genomes have largely retained the complete gene complements inherited from both of the diploid genome ancestors, with at least four PHYA genes and two genes encoding PHYB, PHYC and PHYE in the AD-genomes. We did not identify a PHYD gene in any cotton genomes examined.ConclusionsDetailed sequence analysis suggests that phytochrome genes retained after duplication by segmental duplication and allopolyploidy appear to be evolving independently under a birth-and-death-process with strong purifying selection. Our study provides a preliminary phytochrome gene inventory that is necessary and sufficient for further characterization of the biological functions of each of the cotton phytochrome genes, and for the development of 'candidate gene' markers that are potentially useful for cotton improvement via modern marker-assisted selection strategies.

Highlights

Phytochromes are a family of red/far-red photoreceptors that regulate a number of important developmental traits in cotton (Gossypium spp.), including plant architecture, fiber development, and photoperiodic flowering
Because our results were derived from polymerase chain reaction (PCR), our inventory of the phytochrome gene family in Gossypium spp. is provisional
A majority (>60%) of clones showed the highest similarity in BLAST searches to Arabidopsis PHYE (E value ~ 1e-40)

Summary

Introduction

Phytochromes are a family of red/far-red photoreceptors that regulate a number of important developmental traits in cotton (Gossypium spp.), including plant architecture, fiber development, and photoperiodic flowering. PHYA, PHYB, PHYD and PHYE act partially redundantly in the light-dependent entrainment of the circadian clock [35,36], which in turn regulates transcription of the floral inducer CONSTANS (CO) in a circadian manner [37]. PHYB, PHYD and PHYE act partially redundantly as repressors of flowering that are dependent on R/FR ratio [19,28,30,39]. In this role, PHYB acts downstream of CO as a negative regulator of transcription of the 'florigen' molecule FT (the target of CO) in a tissue specific manner [40]. Genetic variation at the PHYC locus underlies some of the natural phenotypic variation in flowering time in Arabidopsis [42,43]

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