Abstract
A simple and reliable molecular assay to identify sex would be valuable for the management and conservation of critically endangered species such as the antelope Saiga tatarica. To this end, we developed a duplex PCR system to co-amplify the SRY-related HMG box and the ZFX/ZFY genes in a single reaction. The amplification of HMG-box produced a 162 bp amplicon for the male sagas, and none for the female. In contrast, amplification of ZFX/ZFY genes resulted in 739 and 854 bp amplicons for the male and a single 854 bp amplicon for the female. Thus, the duplex PCR assay produced three amplicons for male sagas and one for the female. Blind testing further demonstrated the reliability of this assay system, which could also be used for the sex determination of other herbivores, including buffalo and sheep.
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