Abstract
Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In the second part of the study, we developed a duplex PCR assay for the detection of AAV and CAV which is capable to simultaneously detect both the viral types prevalent in Pakistan with high sensitivity and 100% specificity.
Highlights
IntroductionThe avian adenoviruses are divided into three groups i.e. group Ι, group Π and group III
Adenoviruses are common infectious agents of poultry
Thymus and bone marrow samples were collected from diseased chickens at Grand Parents Laboratory (GP Lab) Lahore, where chickens and other birds are brought for the diagnosis of various diseases from different commercial farms from all over the country
Summary
The avian adenoviruses are divided into three groups i.e. group Ι, group Π and group III. Group Ι is composed of 5 species (A to E) and 12 serotypes (FAdV-1 to FAdV12) of avian adenoviruses of chickens, turkeys, goose and duck [1,2,3]. The group Ι adenoviruses are famous for causing hydropericardium-syndrome in chickens (caused by FAdV-4 strains), Quail Bronchitis in quail (caused by FAdV-1) and inclusion body hepatitis [4,5,6]. Group Π of avian adenoviruses causes hemorrhagic enteritis in turkeys, marble spleen disease in pheasants and splenomegaly in chickens. Group III viruses are famous for egg drop syndrome in chickens and a similar virus is believed to infect duck [8]
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