Duodenal Histomorphological Changes in Broilers Administered poly d, l-lactic-coglycolic acid (PLGA ) Nanoparticles Encapsulated with Peptide

Saja H Al-Rubaee,Ali A Taha,Tahani S Al-Azawi

doi:10.30539/ijvm.v44i1.945

Abstract

This research was carried out to evaluate the effect of poly d, l-lactic-coglycolic acid(PLGA) nanoparticles loaded with peptide (as vaccine) on histomorphological in duodenum of broiler chick. A total of ninety eight, one day old ,unsexed broiler( Rose) chicks were divided randomly into seven groups( 2 replicate in each group) as follows:G1-control,G2 - chicks received traditional vaccine of infectious bursal disease(Volvac@ IBD MLV) ,G3 – Chicks Received PLGA nanoparticles only, G4,G5,G6 and G7 – chicks were received prepared vaccine at 160, 80, 40,and 20 μg of peptide loaded PLGA respectively. At the end of the experiment histopathological examination of duodenum section and histomorphological changes were examined. The histopathological examination of duodenal sections shows an elongation and infiltration of lamina propria (LP) with increase villi height and crypt depth in groups that received PLGA alone and those received peptide loaded PLGA. At the same time these groups reveal an increase in mucosal thickness and in length and width of villi. The histomorphological examination in this study show thicker mucosal layer with deeper crypt in duodenum of broilers that subjected to PLGA administration alone or to different concentration of peptide loaded PLGA compared to G1 and G2. These findings are accompanied by a high density of goblet cells and lower villus hight/crypt depth (V/CD) ratio. All groups show mononuclear cells (MNCs) infiltration in submucosa ofduodenum. In-conclusion, the administration of PLGA nanoparticles is strongly linked to the improvement of the physiological and immunological features of the birds.

Highlights

The field of polymer nanoparticle (PNP) is rapidly growing and playing a necessary role in a varied field of area extending from medicine to biotechnology, conducting material to sensors, electronics and so forth [1,2]
Preparation of PLGA: PLGA nanoparticles encapsulating peptide were prepared by solvent evaporation method [12, 13], with some modification, which can be summarized: 200 mg of PLGA dissolved in 2 ml of DMSO and left at room temperature overnight, PVA prepared by dissolving 2gr in 100 ml of diH2O
At the end of study 2 broiler from each replicate were sacrificed and duodenum collected and after hematoxylin/eosin staining of series section of different parts of the intestinal wall, as described by[14], the mucosal thickness, villus hight, crypt depth and villus hight/crypt depth ratio were measured at low power (10 X)

Summary

Introduction

The field of polymer nanoparticle (PNP) is rapidly growing and playing a necessary role in a varied field of area extending from medicine to biotechnology, conducting material to sensors, electronics and so forth [1,2]. Results and Discussion The results of our experiment in regard to histomorphological parameters (mucosa thickness, villus hight, crypt depth, and villus/crypt ratio) of the duodenum piece in response to ordinary vaccine and PLGA nanoparticles and PLGA loaded with different concentration of peptide (antigenic region) are illustrated in Figure (1, 2, 3,and 4).

Results

Conclusion