Abstract

As a class I carcinogen, aflatoxin B1 (AFB1) contamination in foods and feeds accounts for 75 % of the total mycotoxin contamination. In this work, a simple and reliable surface-enhanced Raman spectroscopy (SERS) method for sensitive and selective detection of AFB1 in peanut samples integrated with dummy molecularly imprinted polymers (DMIPs) is developed. N-isopropylacrylamide (NIPAM) and 7-ethoxycoumarin (7-EOC) are chosen as monomer and dummy template, respectively and their ratio was screened through molecular design in both of kinetic and static adsorption views to form the optimal DMIPs. As-prepared dummy molecularly imprinted solid-phase extraction (DMISPE) could selectively enrich AFB1 from peanut samples. Finally, a liquid–liquid interface self-assembly constructed thioctic acid-decorated AgNPs monolayer film (TA-AgNPs MF) as a SERS-active substrate is employed to determine the amount of AFB1 eluted from DMISPE. SERS assay shows high detection sensitivity for AFB1 in peanut samples with limit of detection of 0.1 μg L−1 and a linear concentration relationship range from 0.1 to 10 μg L−1.

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