Abstract
BackgroundpUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions. The C-terminus of pUL21 in other members of this subfamily has RNA-binding ability; this domain contributes to pseudorabies virus (PRV) retrograde axonal transport in vitro and in vivo and participates in newly replicated viral DNA packaging and intracellular virus transport. However, knowledge regarding duck enteritis virus (DEV) pUL21 is limited.ResultsWe verified that DEV UL21 is a γ2 gene that encodes a structural protein. Moreover, we observed that pUL21 localized to the nucleus and cytoplasm. DEV pUL21 interacted with pUL16 and formed a complex in transfected human embryonic kidney (HEK) 293 T cells and DEV-infected duck embryo fibroblasts (DEFs). These results were further confirmed by CO-IP assays.ConclusionsThe DEV UL21 gene is a late gene, and pUL21 localizes to the nucleus and cytoplasm. DEV UL21 is a virion component. In addition, pUL21 can interact with pUL16. These findings provide insight into the characteristics of UL21 and the interaction between pUL21 and its binding partner pUL16. Our study enhances the understanding of DEV pUL21.
Highlights
PUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions
Preparation of the duck enteritis virus (DEV) UL21 polyclonal antibody To carry out the subsequent experiment, we generated the polyclonal antibody of UL21
A target band of 62 kDa was obtained from infected Duck embryo fibroblast (DEF), which is consistent with the expected size of DEV pUL21 (Fig. 1d)
Summary
PUL21 is a conserved protein of Alphaherpesvirinae that performs multiple important functions. Knowledge regarding duck enteritis virus (DEV) pUL21 is limited. Infection with duck enteritis virus (DEV), a member of the Alphaherpesvirinae subfamily, can cause serious clinical symptoms and pathological changes, such as vascular injury, tissue haemorrhage, gastrointestinal mucosal papulosis-like lesions, and degeneration of lymphoid and parenchymal organs [1,2,3]. The UL21 gene in herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), Marek’s disease virus serotype 2 (MDV-2), and DEV is 1608 bp [7], 1599 bp [8], 1596 bp [9] and 1686 bp [10], respectively.
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