Abstract
Duck enteritis virus (DEV) is a member of the Alphaherpesvirinae subfamily. The characteristics of some DEV genes have been reported. However, information regarding the DEV UL47 gene is limited. In this study, we identified the DEV UL47 gene encoding a late structural protein located in the nucleus of infected cells. We further found that two domains of DEV pUL47, amino acids (aa) 40 to 50 and 768 to 777, could function as nuclear localization sequence (NLS) to guide the nuclear localization of pUL47 and nuclear translocation of heterologous proteins, including enhanced green fluorescent protein (EGFP) and beta-galactosidase (β-Gal). Moreover, pUL47 significantly inhibited polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced interferon beta (IFN-β) production and downregulated interferon-stimulated gene (ISG) expression, such as Mx and oligoadenylate synthetase-like (OASL), by interacting with signal transducer and activator of transcription-1 (STAT1).
Highlights
Duck virus enteritis (DVE), is an acute, septic, and febrile disease that occurs in waterfowl of all ages, with a high mortality rate and a declined duck egg production, resulting in severe economic losses to duck industry [1]
The duck enteritis virus (DEV) UL47 gene is a late gene As shown in Figure 2A, the UL47 gene transcript level increased gradually from 6 hpi to 48 hpi and peaked at 48 hpi followed by a steady decrease
We further identified the expression of the DEV UL47 gene
Summary
Duck virus enteritis (DVE), is an acute, septic, and febrile disease that occurs in waterfowl of all ages, with a high mortality rate and a declined duck egg production, resulting in severe economic losses to duck industry [1]. The herpes simplex virus-1(HSV-1) UL47 gene encodes two major tegument proteins, VP13 and VP14, which represent differently processed forms of posttranslational modification (phosphorylation, glycosylation or nucleotidylylation); VP13/14 is an RNA binding protein and shuttles between the nucleus and cytoplasm [4], modulates the trans-acting activity of trans-inducing. He et al Vet Res (2020) 51:135 factor VP16 and stimulates viral immediate-early gene transcription in newly infected cells [5]. The UL47 genes of HSV-1 [15], ILTV [14], pseudorabies virus (PRV) [16, 17], BoHV-1 [18], varicella-zoster virus (VZV) [19] and Marek’s disease virus (MDV-1) [20] have been demonstrated to be dispensable for viral replication in cell culture
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
