Abstract
BackgroundUL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication.ResultsIn this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins.ConclusionsThe interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.
Highlights
UL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication
Finding Duck enteritis virus (DEV, anatid alphaherpesvirus 1 species), clustered in mardivirus genus, alphaherpesvirinae subfamily, Herpesviridae family according to the latest report of the International Committee on Taxonomy of Viruses (ICTV) [1], causes considerable economic losses to the commercial duck industry and poses a continuous threat to wild and migratory waterfowl populations due to their high mortality and decreased egg production rates [2]
There are three complete genomic sequences of DEV strains available in GenBank: the Chinese virulent DEV strain (DEV CHv) [3, 4], the European virulent strain (2085) [5], and the attenuated vaccine strain (VAC) [6], and the publications related to the
Summary
UL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication. * Correspondence: jiary@sicau.edu.cn; chenganchun@vip.163.com 1Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Full list of author information is available at the end of the article three genome sequences have cast light on the genome structure of DEV. Research on DEV UL24 protein showed that it is located in the cytoplasm around the periphery of the nucleus in DEVinfected DEF cells [32].
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