Abstract
Amplified fragment length polymorphism (AFLP) with multicolored fluorescent molecular markers was used to analyze duck (Anas platyrhynchos) genomic DNA and to construct the first AFLP genetic linkage map. These markers were developed and genotyped in 766 F2 individuals from six families from a cross between two different selected duck lines, brown Tsaiya and Pekin. Two hundred and ninety-six polymorphic bands (64% of all bands) were detected using 18 pairs of fluorescent TaqI/EcoRI primer combinations. Each primer set produced a range of 7 to 29 fragments in the reactions, and generated on average 16.4 polymorphic bands. The AFLP linkage map included 260 co-dominant markers distributed in 32 linkage groups. Twenty-one co-dominant markers were not linked with any other marker. Each linkage group contained three to 63 molecular markers and their size ranged between 19.0 cM and 171.9 cM. This AFLP linkage map provides important information for establishing a duck chromosome map, for mapping quantitative trait loci (QTL mapping) and for breeding applications.
Highlights
Amplified fragment length polymorphism (AFLP) is an application of the DNA fingerprinting technique proposed by Vos et al [1], which is a clever combination of two older methods, restriction fragment length polymorphism (RFLP) [2] and random amplified polymorphic DNA (RAPD) [3,4,5], generating a large number of genetic markers from any genomic DNA [6]
After polymorphism analysis and χ2 tests, 281 AFLP markers obtained from the genomic DNA of six duck families could be used for linkage analysis
One purpose of the resource population produced in this work was to generate individuals with a maximum of heterozygous markers in its F1 generation. This resource population originated from a cross between two genetically different lines: a laying brown Tsaiya line selected for long duration of fertility [36,37] and a Pekin duck line selected as grand parent to produce mule ducks for roasting
Summary
Amplified fragment length polymorphism (AFLP) is an application of the DNA fingerprinting technique proposed by Vos et al [1], which is a clever combination of two older methods, restriction fragment length polymorphism (RFLP) [2] and random amplified polymorphic DNA (RAPD) [3,4,5], generating a large number of genetic markers from any genomic DNA [6]. Linkage maps using AFLP, microsatellite or SNP markers have been established and applied extensively to linkage studies or quantitative trait locus (QTL) mapping in animals such as rats [11], rabbits [12], goats [13], sheep [14], cattle [15], chickens [16,17,18,19,20], turkeys [21], quails [22,23], and fish [24,25] They have been much used for genome mapping, studies on disease resistance and drug (page number not for citation purposes)
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