Abstract
Glutathione coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction methods and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg), with good selectivity over other amino acids. The GSH-Au/AgNCs have two emissions at 616nm and 412nm when excited at 360nm. With the increased concentration of Cys, the ratio of the emission intensities (I616/I412) linearly decreases with Cys inconcentration ranging from 0.05 to 10μM and from 10 to 50μM, respectively. With increased concentrations of Arg, the ratio of I616/I412 linearly decreases with Arg concentration ranging from 0 to 50μM and from 50 to 100μM, respectively. The probe was applied to the determination of Cys and Arg in spiked samples of serum and urine where it gave good recoveries. Graphical abstract Glutathione-coated gold and silver nanoclusters (GSH-Au/AgNCs) were synthesized by one-pot reduction and are found to be viable fluorescent nanoprobes for cysteine (Cys) and arginine (Arg).
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