Dual-emission of a fluorescent graphene oxide–quantum dot nanohybrid for sensitive and selective visual sensor applications based on ratiometric fluorescence

Abstract

A novel nanohybrid ratiometric fluorescence probe comprised of fluorescent graphene oxide and quantum dots (QDs) has been prepared by bringing CdTe QDs of red fluorescence and fluorescent graphene oxide (FGO) of blue fluorescence together through electrostatic attraction and hydrogen bonding interaction between their surface functional groups including carboxyl and amine groups. The nanohybrid ratiometric fluorescence probe exhibits dual emissions at 450 and 650 nm under a single excitation wavelength and shows high sensitivity for the detection of ferrous ions in the presence of H2O2. Ferrous ions reacts with H2O2 to generate very reactive hydroxyl radicals which possess a strong oxidizing nature and easily capture the electrons from the surfaces of the CdTe QDs, leading to fluorescence quenching of the QDs and no effect on the fluorescence of the graphene oxide, which hence results in a great change of the fluorescence ratio. Moreover, the ratiometric fluorescence probe is not only extremely sensitive to ferrous ions, but is also selective over other biologically relevant metal cations. The changes of fluorescence colour ratios can be used for visual sensing applications for ferrous ions in the presence of hydrogen peroxide, and can also be used for the indication of the existence of hydrogen peroxide.