Dual-wavelength spectrophotometric determination of copper in sea-water with α,β,γ,°-tetrakis(1-methylpyridinium-4-yl) porphine

Abstract

Copper has been determined by dual-wavelength spectrophotometry with α,β,γ,°-tetrakis(1-methylpyridinium-4-yl) porphine (TMPyP). The difference in absorbance at the Soret band of the copper complex (423 nm) and that of TMPyP (446 nm) was measured. Copper in a coastal sea-water sample was preconcentrated with a Chelex-100 separation in which l-cysteine was used as eluent. This combination could determine copper at the ng/ml and sub-ng/ml level.