Abstract
The use of absorbance ratio and UV-VIS spectrum scanning techniques is described for the identification and purity check of flavonoid compounds separated by high-performance liquid chromatography (HPLC). The possibility to correlate absorbance ratios. UV spectra and retention times with those of authentic samples greatly enhances the potential of HPLC for qualitative analysis. The techniques were used for the analysis of the major flavonoid compounds found in the plant Uncaria elliptica, namely rutin and (−)-epicatechin. The tissue distribution and the effect of reduced sunlight on the level of these flavonoids were studied. Limitations of our analytical techniques are also discussed.
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