Dual vasoactive effects of tolazoline on rabbit pulmonary arteries

Abstract

To determine the vasoactive effects of tolazoline on isolated rabbit pulmonary arteries. Prospective, in vitro, randomized, controlled trial. Experimental laboratory in a university-affiliated hospital. New Zealand White Rabbits. The pulmonary artery rings were obtained via thoracotomy. Their vasoactive responses were assessed in the presence and absence of intact endothelium and with or without precontraction by norepinephrine (NE, 3 x 10(-6) M) or potassium chloride (KCl, 3 x 10(-2) M). Using a tissue bath preparation, cumulative concentration response curves of tolazoline were obtained at different concentrations (10(-9) to 10(-4) M) after a period of stabilization. Tolazoline caused vasoconstriction of isolated pulmonary arteries without any pretreatment. The magnitude of the constriction was dose related and reached 300 g/g wet tissue at a concentration of 10(-4) M. On KCl-precontracted pulmonary arteries, tolazoline caused significant dose-related vasoconstriction. On the NE-precontracted vessel rings, it elicited significant dose-dependent vasodilation up to 60% relaxation at 10(-5) M. All the above effects were endothelium independent. Tolazoline has dual endothelium-independent vasoactive effects, causing vasoconstriction on isolated rabbit pulmonary arteries, either untreated or precontracted with KCl, and vasodilation on those precontracted with NE. Tolazoline may act as a competitive alpha-adrenoceptor blocking agent.