Abstract
An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
Highlights
Due to their unique optical and electronic properties [1], DNA biosensors based on colloidal gold nanoparticles (AuNPs) show the merits of high sensitivity and selectivity to probe trace amounts of DNA targets, which can fulfil the stringent requirements of clinical genetic diagnosis, drug discovery, and the detection of environmentally hazardous compounds [2]
Afterwards, the probes self-organize into a constrained conformation on the nanoparticle surface and the fluorophore dye is quenched by the particle [6]
To detect DNA hybridization, AuNPs coated with streptavidin were selected as fluorescence quenchers
Summary
Due to their unique optical and electronic properties [1], DNA biosensors based on colloidal gold nanoparticles (AuNPs) show the merits of high sensitivity and selectivity to probe trace amounts of DNA targets, which can fulfil the stringent requirements of clinical genetic diagnosis, drug discovery, and the detection of environmentally hazardous compounds [2]. Afterwards, the probes self-organize into a constrained conformation on the nanoparticle surface and the fluorophore dye is quenched by the particle [6]. The constrained conformation opens and the fluorophore is separated from the particle surface, restoring luminescence. These phenomena qualify AuNPs as highly interesting substrates for rapid and sensitive detections of target oligonucleotides via fluorescence quenching between the particles and DNA molecules [7]. Biotin labeled probe DNA act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversible detection of DNA hybridization is accomplished
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
