Dual progesterone action in diencephalon facilitates the induction of sexual receptivity in estrogen-primed golden hamsters

Abstract

In order to identify and characterize the progesterone (P) sensitive neural system that regulates feminine sexual behavior, 28-gauge P-filled cannulae were implanted in the medial preoptic area (MPO), ventromedial hypothalamus (VMH), and central gray (CG) of ovariectomized estrogen-primed golden hamsters. Dual implants of P were placed either ipsilaterally or contralaterally in brain sites consisting of MPO-VMH, MPO-CG, or VMH-CG combinations. Tests for sexual receptivity commenced 44 hr after estrogen priming and consisted of a preimplantation test followed 4.5 to 5.5 hr later by a postimplantation test. In the preimplantation test, stimulus males were attacked when placed into the female's home cage which indicated that the subsequent display of sexual receptivity occurring in the postimplantation test was due to the action of P. Dual implants of P placed either ipsilaterally or contralaterally in MPO-VMH regions were significantly more effective in facilitating lordosis behavior than dual P implants placed in MPO-CG or VMH-CG regions. However, the duration of lordotic responsiveness produced by dual P implants in MPO and VMH regions appears to be shorter than the duration of lordosis typically observed in intact females on proestrus. Results suggest that MPO and VMH regions are sensitive to the lordosis facilitating actions of small dual implants of P.