Dual-mode sensing platform based on aptamer-tunable catalytic activity of mesoporous polydopamine/MnO2 nanozymes for detecting S. aureus

Abstract

Staphylococcus aureus (S. aureus) infections pose a serious threat to human health worldwide. Therefore, selective and accurate detection of S. aureus is necessary for preventing infections. Herein, we design the colorimetric-electrochemical sensing platform based on specific oligonucleotide (SA31 aptamer)-tunable oxidase-like activity of mesoporous polydopamine/MnO2 (MPDA/MnO2) nanozymes for the S. aureus detection. The MPDA/MnO2 nanozymes possess excellent oxidase-like activity and electrochemical property. MPDA/MnO2 nanozymes can catalyze the oxidation of TMB to generate TMB+ (blue color) and TMB2+ (yellow color) products. The oxidase-like activity of MPDA/MnO2 can be inhibited when the SA31 is adsorbed on the surface of the nanozymes. In the presence of S. aureus, SA31 aptamer desorbs from MPDA/MnO2 surface based on the specific identification between S. aureus and aptamer, resulting in the recovery of their oxidase-like activity. Based on the different oxidative degree of TMB, the color of sensing solution changes from blue to yellow, the electrochemical signals also have changed simultaneously. The colorimetric-electrochemical sensing platform can accurately detect S. aureus, showing a low detection limit in 3 CFU/mL (S/N = 3). Furthermore, the dual-mode sensing platform can be utilized for the accurate and specific detection of S. aureus in real sample with the recovery of 95.15%− 115.28%. This work based on SA31 aptamer-tunable oxidase-like activity of MPDA/MnO2 nanozymes provides a dual-mode assay for specific and accurate detection of S. aureus.